Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
نویسندگان
چکیده
منابع مشابه
identification and sequencing of candida krusei aconitate hydratase gene using rapid amplification of cdna ends method and phylogenetic analysis
conclusions results of the current study indicated that aconitase was not a suitable target to design new anti-fungal drugs that selectively block this enzyme. materials and methods candida krusei (atcc: 6258) aconitase gene was determined by 5’rapid amplification of cdna ends (race) method and degenerate polymerase chain reaction (pcr) and analyzed using bioinformatics softwares. objectives th...
متن کاملIdentification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
BACKGROUND The production and development of an effective fungicidal drug requires the identification of an essential fungal protein as a drug target. Aconitase (ACO) is a mitochondrial protein that plays a vital role in tricarboxylic acid (TCA) cycle and thus production of energy within the cell. OBJECTIVES The current study aimed to sequence Candida krusei ACO gene and determine any amino a...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2015
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.25218